Newswise — CLEVELAND—A team of researchers from the Case Western Reserve University School of Medicine has developed a new method for target DNA sequence amplification, testing and analysis. This new technique, or reaction, known as AMPLON (Amplifying DNA with Multiarm Priming and Looping Optimization of Nucleic Acid), offers an alternative to the previously accepted “gold-standard” Polymerase Chain Reaction (PCR) method, opening the opportunity for more applications in medical diagnosis. The team’s findings were recently published in the journal Advanced Materials .
“AMPLON has the potential to positively change the way molecular analysis and clinical diagnostics are performed,” said Mohamed S. Draz, an assistant professor at the School of Medicine and the study’s principal investigator, “from infectious-disease diagnostics to personalized medicine and environmental monitoring.” How it works Researchers use such technology to compare the DNA of sick cells to that of healthy cells, allowing them to better understand the changes that occur as a disease progresses and how to treat it.
AMPLON provides several extensions along the DNA strand to simultaneously increase the speed and accuracy of DNA synthesis under constant temperature conditions. Using this new simplified process eliminates the need to operate between high and low temperature extremes that can cause stress on materials. It also makes the amplification process more structured and accessible, esp.
